Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci
نویسندگان
چکیده
منابع مشابه
Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci.
The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polysaccharide, in multiple group B streptococcus serotypes, all of which elaborate...
متن کاملSynthesis of N-acetylneuraminic acid and of CMP-N-acetylneuraminic acid in the rat liver cell.
Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...
متن کاملCMP-N-acetylneuraminic acid synthetase interacts with fragile X related protein 1
Fragile X mental retardation protein (FMRP), fragile X related 1 protein (FXR1P) and FXR2P are the members of the FMR protein family. These proteins contain two KH domains and a RGG box, which are characteristic of RNA binding proteins. The absence of FMRP, causes fragile X syndrome (FXS), the leading cause of hereditary mental retardation. FXR1P is expressed throughout the body and important f...
متن کاملCMP - N - acetylneuraminic a id synthetase of Escherichia coliz high level expression , purification and use in
The gene encoding CIIP-A'-acet\ ' lneuraminic acid (CIIPNeuAc) s1'nthetase (EC 2.7.7.43) in Escherichia coli serot!'pe 07 Kl w'as isolated and overexpressed in E.coli W3ll0. \Iaximum expression of 8-1Ooh of the soluble E.coli protein rras achieved b1'placing the gene rr i th an engineered 5'-terminus and Shine-Dalgarno sequence into a pKK223 rector derivative behind the tac promoter. The overex...
متن کاملCorrigendum: Characterization of a novel N-acetylneuraminic acid lyase favoring industrial N-acetylneuraminic acid synthesis process
The original version of this article contained an error in testing the kinetic parameters of CgNal towards pyruvate, in which the concentration of ManNAc (50 mM) was not in excess. This error was corrected by re-running the assay in the presence of excessive ManNAc (180 mM). The corrected kinetic parameters of CgNal towards pyruvate are shown in Table 1. These changes do not change the conclusi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1994
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.176.23.7372-7374.1994